Publications
Publications
2024
A Novel RNA Aptamer as Synthetic Inducer of DasR Controlled Transcription
In: ACS Synthetic Biology 13 (2024), S. 319-327
ISSN: 2161-5063
DOI: 10.1021/acssynbio.3c00553
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2023
An antiviral targeting strategy based on the inducible interference with cytomegalovirus nuclear egress complex
In: Antiviral Research 212 (2023), Art.Nr.: 105557
ISSN: 0166-3542
DOI: 10.1016/j.antiviral.2023.105557
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De novo prediction of explicit water molecule positions by a novel algorithm within the protein design software MUMBO
In: Scientific Reports 13 (2023), S. 16680-
ISSN: 2045-2322
DOI: 10.1038/s41598-023-43659-w
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2022
The Coxiella burnetii T4SS effector protein AnkG hijacks the 7SK small nuclear ribonucleoprotein complex for reprogramming host cell transcription
In: PLoS Pathogens 18 (2022), Art.Nr.: e1010266
ISSN: 1553-7366
DOI: 10.1371/journal.ppat.1010266
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The crystal structure of the varicella-zoster Orf24-Orf27 nuclear egress complex spotlights multiple determinants of herpesvirus subfamily specificity
In: Journal of Biological Chemistry 298 (2022), Art.Nr.: 101625
ISSN: 0021-9258
DOI: 10.1016/j.jbc.2022.101625
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2021
Exploring the Human Cytomegalovirus Core Nuclear Egress Complex as a Novel Antiviral Target: A New Type of Small Molecule Inhibitors
In: Viruses 13 (2021)
ISSN: 1999-4915
DOI: 10.3390/v13030471
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Crystal structures of adenylylated and unadenylylated PII protein GlnK from Corynebacterium glutamicum
In: Acta Crystallographica Section D : Structural Biology 77 (2021), S. 325-335
ISSN: 2059-7983
DOI: 10.1107/S2059798321000735
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A PROSS-designed extensively mutated estrogen receptor α variant displays enhanced thermal stability while retaining native allosteric regulation and structure
In: Scientific Reports 11 (2021), Art.Nr.: 10509
ISSN: 2045-2322
DOI: 10.1038/s41598-021-89785-1
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Cytomegalovirus immediate-early 1 proteins form a structurally distinct protein class with adaptations determining cross-species barriers
In: PLoS Pathogens 17 (2021)
ISSN: 1553-7366
DOI: 10.1371/journal.ppat.1009863
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2020
The complex formed between a synthetic RNA aptamer and the transcription repressor TetR is a structural and functional twin of the operator DNA-TetR regulator complex
In: Nucleic Acids Research 48 (2020), S. 3366-3378
ISSN: 0305-1048
DOI: 10.1093/nar/gkaa083
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The CD83 Molecule – An Important Immune Checkpoint
In: Frontiers in Immunology 11 (2020), Art.Nr.: 721
ISSN: 1664-3224
DOI: 10.3389/fimmu.2020.00721
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Recombinant protein production and purification of SiiD, SiiE and SiiF - Components of the SPI4-encoded type I secretion system from Salmonella Typhimurium
In: Protein Expression and Purification 172 (2020), Art.Nr.: 105632
ISSN: 1046-5928
DOI: 10.1016/j.pep.2020.105632
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Nuclear Egress Complexes of HCMV and Other Herpesviruses: Solving the Puzzle of Sequence Coevolution, Conserved Structures and Subfamily-Spanning Binding Properties
In: Viruses 12 (2020)
ISSN: 1999-4915
DOI: 10.3390/v12060683
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High-resolution crystal structures of two prototypical β- And γ-herpesviral nuclear egress complexes unravel the determinants of subfamily specificity
In: Journal of Biological Chemistry 295 (2020), S. 3189-3201
ISSN: 0021-9258
DOI: 10.1074/jbc.RA119.011546
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2019
siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
In: Journal of Visualized Experiments (2019)
ISSN: 1940-087X
DOI: 10.3791/60190
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NewBG: A surrogate corticosteroid-binding globulin with an unprecedentedly high ligand release efficacy
In: Journal of Structural Biology 207 (2019), S. 169-182
ISSN: 1047-8477
DOI: 10.1016/j.jsb.2019.05.006
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Structural and functional characterization of SiiA, an auxiliary protein from the SPI4-encoded type 1 secretion system from Salmonella enterica
In: Molecular Microbiology (2019)
ISSN: 0950-382X
DOI: 10.1111/mmi.14368
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Manipulation of charge distribution in the arginine and glutamate clusters of the OmpG pore alters sugar specificity and ion selectivity
In: Biochimica Et Biophysica Acta-Biomembranes 1861 (2019), Art.Nr.: 183021
ISSN: 0005-2736
DOI: 10.1016/j.bbamem.2019.07.009
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2018
Near-native, site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis.
In: Scientific Reports 8 (2018), S. 4977
ISSN: 2045-2322
DOI: 10.1038/s41598-018-23154-3
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Hydration Structure and Dynamics of Inhibitor-Bound HIV-1 Protease.
In: Journal of Chemical Theory and Computation 14 (2018), S. 2784-2796
ISSN: 1549-9618
DOI: 10.1021/acs.jctc.8b00097
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Design of an allosterically modulated doxycycline and doxorubicin drug-binding protein.
In: Proceedings of the National Academy of Sciences of the United States of America (2018)
ISSN: 0027-8424
DOI: 10.1073/pnas.1716666115
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PRISEs (progesterone 5β-reductase and/or iridoid synthase-like 1,4-enone reductases): Catalytic and substrate promiscuity allows for realization of multiple pathways in plant metabolism.
In: Phytochemistry 156 (2018), S. 9-19
ISSN: 0031-9422
DOI: 10.1016/j.phytochem.2018.08.012
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2017
Crystal Structure of the Extracellular Domain of the Human Dendritic Cell Surface Marker CD83
In: Journal of Molecular Biology 429 (2017), S. 1227-1243
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2017.03.009
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The human cytomegalovirus nuclear egress complex unites multiple functions: Recruitment of effectors, nuclear envelope rearrangement, and docking to nuclear capsids
In: Reviews in Medical Virology 27 (2017), Art.Nr.: e1934
ISSN: 1099-1654
DOI: 10.1002/rmv.1934
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Structural and functional dissection reveals distinct roles of Ca2+-binding sites in the giant adhesin SiiE of Salmonella enterica
In: PLoS Pathogens 13 (2017), Art.Nr.: e1006418
ISSN: 1553-7366
DOI: 10.1371/journal.ppat.1006418
URL: https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006418
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Structural and functional dissection reveals distinct roles of Ca2+-binding sites in the giant adhesin SiiE of Salmonella enterica
In: PLoS Pathogens 13 (2017), Art.Nr.: e1006418
ISSN: 1553-7366
DOI: 10.1371/journal.ppat.1006418
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The human cytomegalovirus IE1 protein antagonizes PML nuclear body-mediated intrinsic immunity via the inhibition of PML de novo SUMOylation
In: Journal of Virology 91 (2017), Art.Nr.: e02049-16
ISSN: 0022-538X
DOI: 10.1128/JVI.02049-16
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Nuclear trafficking of the anti-apoptotic Coxiella burnetii effector protein AnkG requires binding to p32 and Importin-alpha 1
In: Cellular Microbiology 19 (2017), S. e12634-
ISSN: 1462-5814
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Similarities in the structure of the transcriptional repressor AmtR in two different space groups suggest a model for the interaction with GlnK
In: Acta Crystallographica Section:F Structural Biology Communications 73 (2017), S. 146-151
ISSN: 2053-230X
DOI: 10.1107/S2053230X17002485
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2016
Crystal Structures of the Global Regulator DasR from Streptomyces coelicolor: Implications for the Allosteric Regulation of GntR/HutC Repressors.
In: PLoS ONE 11 (2016), S. e0157691
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0157691
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Expression, crystallization and structure elucidation of γ-terpinene synthase from Thymus vulgaris
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications 72 (2016), S. 16-23
ISSN: 1744-3091
DOI: 10.1107/S2053230X15023043
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Nuclear trafficking of the anti-apoptotic Coxiella burnetii effector protein AnkG requires binding to p32 and Importin-α1
In: Cellular Microbiology 19 (2016)
ISSN: 1462-5814
DOI: 10.1111/cmi.12634
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2015
Structural insight into operator dre-sites recognition and effector binding in the GntR/HutC transcription regulator NagR
In: Nucleic Acids Research 43 (2015), S. 1283-1296
ISSN: 0305-1048
DOI: 10.1093/nar/gku1374
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Controlled crystal dehydration triggers a space-group switch and shapes the tertiary structure of cytomegalovirus immediate-early 1 (IE1) protein
In: Acta Crystallographica Section D: Biological Crystallography 71 (2015), S. 1493-1504
ISSN: 1399-0047
DOI: 10.1107/S1399004715008792
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Characterization of recombinant human cytomegaloviruses encoding IE1 mutants L174P and 1-382 reveals that viral targeting of PML bodies perturbs both intrinsic and innate immune responses
In: Journal of Virology 90 (2015), S. 1190-1205
ISSN: 0022-538X
DOI: 10.1128/JVI.01973-15
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Multiple allosteric effectors control the affinity of DasR for its target sites
In: Biochemical and Biophysical Research Communications 464 (2015), S. 324-329
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2015.06.152
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Crystal structure of the human cytomegalovirus pUL50-pUL53 core nuclear egress complex provides insight into a unique assembly scaffold for virus-host protein interactions
In: Journal of Biological Chemistry 290 (2015), S. 27452-27458
ISSN: 0021-9258
DOI: 10.1074/jbc.C115.686527
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2014
Antiapoptotic activity of Coxiella burnetii effector protein AnkG is controlled by p32-dependent trafficking
In: Infection and Immunity 82 (2014), S. 2763-2771
ISSN: 0019-9567
DOI: 10.1128/IAI.01204-13
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Truncation of N-terminal regions of Digitalis lanata progesterone 5β-reductase alters catalytic efficiency and substrate preference
In: Biochimie 101 (2014), S. 31-38
ISSN: 0300-9084
DOI: 10.1016/j.biochi.2013.12.010
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Crystal structure of cytomegalovirus IE1 protein reveals targeting of TRIM family member PML via coiled-coil interactions
In: PLoS Pathogens 10 (2014), S. e1004512
ISSN: 1553-7366
DOI: 10.1371/journal.ppat.1004512
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Structural Basis for the Recognition of Human Cytomegalovirus Glycoprotein B by a Neutralizing Human Antibody
In: PLoS Pathogens 10 (2014), S. e1004377
ISSN: 1553-7366
DOI: 10.1371/journal.ppat.1004377
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Helicobacter pylori CagL Y58/E59 mutation turns-off type IV secretion-dependent delivery of CagA into host cells
In: PLoS ONE 9 (2014), Art.Nr.: e97782
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0097782
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2013
Identification of a Novel TGF-β-Binding Site in the Zona Pellucida C-terminal (ZP-C) Domain of TGF-β-Receptor-3 (TGFR-3)
In: PLoS ONE 8 (2013), Art.Nr.: e67214
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0067214
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Structural insight into the giant Ca²?-binding adhesin SiiE: implications for the adhesion of Salmonella enterica to polarized epithelial cells
In: Structure 21 (2013), S. 741-52
ISSN: 0969-2126
DOI: 10.1016/j.str.2013.02.020
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Unexpected features in the Protein Data Bank entries 3qd1 and 4i8e: The structural description of the binding of the serine-rich repeat adhesin GspB to host cell carbohydrate receptor is not a solved issue
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications 69 (2013), S. 1071-1076
ISSN: 1744-3091
DOI: 10.1107/S1744309113014383
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Development of a yeast-based screening system for the identification of allergenic determinants of profilins
In: Allergy 68 (2013), S. 135-135
ISSN: 0105-4538
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Characterization of a discontinuous neutralizing epitope on glycoprotein B of human cytomegalovirus
In: Journal of Virology 87 (2013), S. 8927-39
ISSN: 0022-538X
DOI: 10.1128/JVI.00434-13
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2012
Corticosteroid-Binding Globulin: Structure-Function Implications from Species Differences
In: PLoS ONE 7 (2012), Art.Nr.: e52759
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0052759
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Purification, crystallization and preliminary X-ray diffraction analysis of the Hsp40 protein CPIP1 from Nicotiana tabacum
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications 68 (2012), S. 236-239
ISSN: 1744-3091
DOI: 10.1107/S1744309111055928
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The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi's sarcoma-associated herpesvirus
In: Nature Medicine 18 (2012), S. 961-966
ISSN: 1078-8956
DOI: 10.1038/nm.2805
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Redirecting T cells to ewing's sarcoma family of tumors by a chimeric NKG2D receptor expressed by lentiviral transduction or mRNA transfection
In: PLoS ONE 7 (2012), Art.Nr.: e31210
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0031210
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Specific residues of a conserved domain in the N terminus of the human cytomegalovirus pUL50 protein determine its intranuclear interaction with pUL53
In: Journal of Biological Chemistry 287 (2012), S. 24004-16
ISSN: 0021-9258
DOI: 10.1074/jbc.M111.331207
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Hypoallergenic profilin - A new way to identify allergenic determinants
In: Febs Journal 279 (2012), S. 2727-2736
ISSN: 1742-464X
DOI: 10.1111/j.1742-4658.2012.08656.x
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2010
Highly conserved progesterone 5β-reductase (P5βR) genes, from 5β-cardenolide-free and 5β-cardenolide-producing angiosperms.
In: Phytochemistry 71 (2010), S. 1495-1505
ISSN: 0031-9422
DOI: 10.1016/j.phytochem.2010.06.004
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2009
- Hasselt K, Sevvana M, Burkovski A, Muller, Y:
Crystallization and preliminary analysis of selenomethionine-labelled AmtR from Corynebacterium glutamicum
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications 65 (2009), S. 1123-1127
ISSN: 1744-3091
DOI: 10.1107/S174430910903663X
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The VEP1 gene (At4g24220) encodes a short-chain dehydrogenase/reductase with 3-oxo-Δ4,5-steroid 5β-reductase activity in Arabidopsis thaliana L.
In: Biochimie 91 (2009), S. 517-525
ISSN: 0300-9084
DOI: 10.1016/j.biochi.2008.12.005
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Structural origins for selectivity and specificity in an engineered bacterial repressor-inducer pair
In: Febs Journal 276 (2009), S. 5610-5621
ISSN: 1742-464X
DOI: 10.1111/j.1742-4658.2009.07254.x
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2008
A protein functional leap: how a single mutation reverses the function of the transcription regulator TetR
In: Nucleic Acids Research 36 (2008), S. 4390-4401
ISSN: 0305-1048
DOI: 10.1093/nar/gkn400
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The crystal structure of progesterone 5beta-reductase from Digitalis lanata defines a novel class of short-chain dehydrogenases/reductases
In: Journal of Biological Chemistry 283 (2008), S. 17260-17269
ISSN: 0021-9258
DOI: 10.1074/jbc.M706185200
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2007
A glucose kinase from Mycobacterium smegmatis
In: Journal of Molecular Microbiology and Biotechnology 12 (2007), S. 75-81
ISSN: 1464-1801
DOI: 10.1159/000096462
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2006
Crystallization and preliminary crystallographic analysis of selenomethoionine-labelled progesterone 5β-reductase from Digitalis lanata Ehrh.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications F62 (2006), S. 186-188
ISSN: 1744-3091
DOI: 10.1107/S1744309106001916
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MUMBO: a protein-design approach to crystallographic model building and refinement.
In: Acta Crystallographica Section D: Biological Crystallography 62 (2006), S. 648-658
ISSN: 0907-4449
DOI: 10.1107/S0907444906013333
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